Expression of ORF2 protein of TTV for development of EIA system
نویسندگان
چکیده
منابع مشابه
Expression of ORF2 protein of TTV for development of EIA system
Methods ORF2 region of TTV genotype1 (JA20 isolate) was amplified using gene specific primers and cloned in pET19b expression vector for protein expression. Expressed protein was confirmed by western blotting using anti-His antibodies against N-terminal 6XHis tag of expressed protein. Antigenicity of ORF2 was determined by western blot, detecting anti-TTV antibodies in 50 human sera of which, 1...
متن کاملExpression of TTV-ORF2 Protein for Detection of Anti-TTV IgG Antibodies in Human Sera
The present study describes the cloning and expression of ORF-2 region of TTV genome and the use of expressed peptide in developing immunoassay for detection of anti-TTV antibodies in serum. Presence of TTV-DNA in serum was detected by PCR amplifying N-22 region of ORF-1 of TTV genome. This was followed by genotyping of TTV by RFLP using N-22 amplicon. Using genotype-1 positive serum as the sou...
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ژورنال
عنوان ژورنال: BMC Infectious Diseases
سال: 2012
ISSN: 1471-2334
DOI: 10.1186/1471-2334-12-s1-p68